rabbit anti pex14 polyclonal antibody
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Proteintech
rabbit anti pex14 polyclonal antibody
Rabbit Anti Pex14 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and morehttps://www.bioz.com/product/10594+1+ap/PEX14+Antibody/pmc13657169-6-0-5
Average 94 stars, based on 93 article reviews
rabbit anti pex14 polyclonal antibody - by
Bioz Stars,
2026-10
94/100 stars
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1) Product Images from "Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells"
Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells
Journal: iScience
doi: 10.1016/j.isci.2026.117658
Figure Legend Snippet: Deletion of Pex19 impairs the growth of mESCs (A) Pex19 deficiency reduces the growth of mESCs. Equal numbers of WT and Pex19 KO 46C mESCs were seeded and cultured in ESGRO medium, and cell counts were analyzed on day 3 ( n = 3 biological replicates/group; data are presented as mean ± SEM; Student’s t test, ∗∗ p < 0.01). (B) Pex19 deficiency reduces the single-cell self-renewal capacity of mESCs. WT and Pex19 KO mESCs were cultured for colony formation for 5 days ( n = 3 biological replicates/group; data are presented as mean ± SEM, Student’s t test, ∗ p < 0.05). (C) Pex19 deficiency increases cell death and apoptosis of mESCs ( n = 3 biological replicates/group, Student’s t test, ∗∗ p < 0.01, ∗∗∗ p < 0.001). (D) Adding back PEX19 restores the peroxisomal targeting of PEX14. Scale bars, 5 μm. (E–G) Adding back PEX19 rescues defective cell growth and elevated cell death in Pex19 KO mESCs. E and F, n = 3–4 biological replicates/group; data are presented as mean ± SEM, one-way ANOVA, ∗q < 0.05, ∗∗q < 0.01, ∗∗∗q < 0.001, n.s., not significant). (H) Top enriched GSEA pathways in Pex19 KO vs. WT mESCs. Transcriptomes of WT and Pex19 KO 46C mESCs from the RNA-seq dataset were analyzed using GSEA as described in . NES, Normalized Enrichment Score; FDR, False Discovery Rate. See also .
Techniques Used: Cell Culture, Single Cell, Colony Assay, RNA Sequencing
Figure Legend Snippet:
Techniques Used: Cell-Signaling, Magnetic Beads, Virus, Recombinant, ROS Assay, Reverse Transcription, SYBR Green Assay, Staining, Plasmid Preparation, Flow Cytometry, Western Blot, Expressing, Control, Software
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Fluorescence:Article Title: Upregulated pexophagy limits the capacity of selective autophagy
Article Snippet: .. Target Product Number Dilution Immuno- fluorescence Western Blot PRIMARY ANTIBODIES PMP70 Abcam; ab3421 1:2000 1:1000 PEX1 BD Biosciences; 611719 1:250 PEX13 Abcam; ab96841 1:1000 PEX14 Proteintech; 10594-1-AP 1:5000 ATG12 Cell Signaling; 2010 1:500 NBR1 Abnova; H00004077-MOI 1:500 MAP1LC3B Thermo Fisher Scientific; A-21070 1:1000 1:1000 SQSTM1 BD Biosciences; 610832 1:1000 OPTN Novus Biologicals; NBPP1-84682 1:1000 CVα Abcam; ab14748 1:5000 MFN2 Abcam; ab56889 1:1000 HSP60 Abcam; ab46798 1:2000 1:10,000 FK2 Enzo; BML-PW8810 1:200 FK1 Millipore Sigma; 04-262 1:200 LAMP1A DSHB; H5C6 1:1000 ATG13 Cell Signaling; 13468 1:1000 phos-ATG13 Thermo Fisher Scientific; 600-401- C49S 1:1000 BNIP3L/Nix Cell Signaling; 12396 1:1000 mTOR Cell Signaling; 2983 1:1000 phos-mTOR Cell Signaling; 2971 1:1000 p70 S6K Cell Signaling; 2708 1:1000 phos-p70 S6K Cell Signaling; 9205 1:1000 FIP200 Cell Signaling; 12436 1:1000 FLAG (WB) Millipore Sigma; F3165 1:8000 FLAG (IF) Millipore Sigma; F7425 1:1000 α-Synuclein BD Biosciences; 610786 1:1000 Catalase Millipore Sigma; 219010 1:5000 HTT Millipore Sigma; MAB2166 1:1000 1:1000 ULK1 Cell Signaling; 8054 1:1000 phos-ULK1 Cell Signaling; 14202 1:1000 SECONDARY ANTIBODIES GAPDH-HRP Novus Biologicals; NB300-328H 1:10,000 β-Actin-HRP Cell Signaling; 5125 1:10,000 Vinculin-HRP Cell Signaling; 18799 1:10,000 anti-rabbit IgG-HRP Thermo Scientific; 31460 1:10,000 anti-mouse IgG-HRP Cedarlane; CLCC30007 1:10,000 anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific; A11001 1:1000 anti-rabbit IgG Alexa Fluor 568 Thermo Fisher Scientific; A-11011 1:1000 anti-mouse IgG Alexa Fluor 568 Thermo Fisher Scientific; A-11004 1:1000 anti-mouse IgG Alexa Fluor 647 Thermo Fisher Scientific; A-31571 1:1000 Supplementary Data Figure 1. ..
Western Blot:Article Title: Upregulated pexophagy limits the capacity of selective autophagy
Article Snippet: .. Target Product Number Dilution Immuno- fluorescence Western Blot PRIMARY ANTIBODIES PMP70 Abcam; ab3421 1:2000 1:1000 PEX1 BD Biosciences; 611719 1:250 PEX13 Abcam; ab96841 1:1000 PEX14 Proteintech; 10594-1-AP 1:5000 ATG12 Cell Signaling; 2010 1:500 NBR1 Abnova; H00004077-MOI 1:500 MAP1LC3B Thermo Fisher Scientific; A-21070 1:1000 1:1000 SQSTM1 BD Biosciences; 610832 1:1000 OPTN Novus Biologicals; NBPP1-84682 1:1000 CVα Abcam; ab14748 1:5000 MFN2 Abcam; ab56889 1:1000 HSP60 Abcam; ab46798 1:2000 1:10,000 FK2 Enzo; BML-PW8810 1:200 FK1 Millipore Sigma; 04-262 1:200 LAMP1A DSHB; H5C6 1:1000 ATG13 Cell Signaling; 13468 1:1000 phos-ATG13 Thermo Fisher Scientific; 600-401- C49S 1:1000 BNIP3L/Nix Cell Signaling; 12396 1:1000 mTOR Cell Signaling; 2983 1:1000 phos-mTOR Cell Signaling; 2971 1:1000 p70 S6K Cell Signaling; 2708 1:1000 phos-p70 S6K Cell Signaling; 9205 1:1000 FIP200 Cell Signaling; 12436 1:1000 FLAG (WB) Millipore Sigma; F3165 1:8000 FLAG (IF) Millipore Sigma; F7425 1:1000 α-Synuclein BD Biosciences; 610786 1:1000 Catalase Millipore Sigma; 219010 1:5000 HTT Millipore Sigma; MAB2166 1:1000 1:1000 ULK1 Cell Signaling; 8054 1:1000 phos-ULK1 Cell Signaling; 14202 1:1000 SECONDARY ANTIBODIES GAPDH-HRP Novus Biologicals; NB300-328H 1:10,000 β-Actin-HRP Cell Signaling; 5125 1:10,000 Vinculin-HRP Cell Signaling; 18799 1:10,000 anti-rabbit IgG-HRP Thermo Scientific; 31460 1:10,000 anti-mouse IgG-HRP Cedarlane; CLCC30007 1:10,000 anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific; A11001 1:1000 anti-rabbit IgG Alexa Fluor 568 Thermo Fisher Scientific; A-11011 1:1000 anti-mouse IgG Alexa Fluor 568 Thermo Fisher Scientific; A-11004 1:1000 anti-mouse IgG Alexa Fluor 647 Thermo Fisher Scientific; A-31571 1:1000 Supplementary Data Figure 1. ..
Cell-Signaling:Article Title: Upregulated pexophagy limits the capacity of selective autophagy
Article Snippet: .. Target Product Number Dilution Immuno- fluorescence Western Blot PRIMARY ANTIBODIES PMP70 Abcam; ab3421 1:2000 1:1000 PEX1 BD Biosciences; 611719 1:250 PEX13 Abcam; ab96841 1:1000 PEX14 Proteintech; 10594-1-AP 1:5000 ATG12 Cell Signaling; 2010 1:500 NBR1 Abnova; H00004077-MOI 1:500 MAP1LC3B Thermo Fisher Scientific; A-21070 1:1000 1:1000 SQSTM1 BD Biosciences; 610832 1:1000 OPTN Novus Biologicals; NBPP1-84682 1:1000 CVα Abcam; ab14748 1:5000 MFN2 Abcam; ab56889 1:1000 HSP60 Abcam; ab46798 1:2000 1:10,000 FK2 Enzo; BML-PW8810 1:200 FK1 Millipore Sigma; 04-262 1:200 LAMP1A DSHB; H5C6 1:1000 ATG13 Cell Signaling; 13468 1:1000 phos-ATG13 Thermo Fisher Scientific; 600-401- C49S 1:1000 BNIP3L/Nix Cell Signaling; 12396 1:1000 mTOR Cell Signaling; 2983 1:1000 phos-mTOR Cell Signaling; 2971 1:1000 p70 S6K Cell Signaling; 2708 1:1000 phos-p70 S6K Cell Signaling; 9205 1:1000 FIP200 Cell Signaling; 12436 1:1000 FLAG (WB) Millipore Sigma; F3165 1:8000 FLAG (IF) Millipore Sigma; F7425 1:1000 α-Synuclein BD Biosciences; 610786 1:1000 Catalase Millipore Sigma; 219010 1:5000 HTT Millipore Sigma; MAB2166 1:1000 1:1000 ULK1 Cell Signaling; 8054 1:1000 phos-ULK1 Cell Signaling; 14202 1:1000 SECONDARY ANTIBODIES GAPDH-HRP Novus Biologicals; NB300-328H 1:10,000 β-Actin-HRP Cell Signaling; 5125 1:10,000 Vinculin-HRP Cell Signaling; 18799 1:10,000 anti-rabbit IgG-HRP Thermo Scientific; 31460 1:10,000 anti-mouse IgG-HRP Cedarlane; CLCC30007 1:10,000 anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific; A11001 1:1000 anti-rabbit IgG Alexa Fluor 568 Thermo Fisher Scientific; A-11011 1:1000 anti-mouse IgG Alexa Fluor 568 Thermo Fisher Scientific; A-11004 1:1000 anti-mouse IgG Alexa Fluor 647 Thermo Fisher Scientific; A-31571 1:1000 Supplementary Data Figure 1. ..
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